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Journal of Forensic Identification

JFI Article Abstracts from 2022 are available to view here at this time

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JFI Abstracts from 2022

Issue 1: April - June 2022 

Y-STR Data Leads to Individualization of Accused in a Sensational Rape and Murder Case

Author(s): Chauhan, Kamal; Mohapatra1, B. K.; Sharma, Anchal; Dagar, Seema B. K. Mohapatra; Bhandari, Deepika; Sahajpal, Vivek
Type: Case Report
Published: 2022, Volume 72, Issue 2, Page 133
Abstract: This case report discusses the investigation of a rape and murder case. The Y-STR DNA profile of the brother and the autosomal STR DNA profile of the mother of the suspected perpetrator (who had absconded) were used to identify the perpetrator. This case highlights the absence of a criminal DNA database in India, where it becomes extremely difficult to solve cases and track repeat offenders in a population of more than 1.35 billion (17.7% of the world’s population).

Choosing a Forensic Quantification Kit to Optimize DNA Profiling Workflow

Author(s): Spitzer, Aya; Einot, Naftaly; Voskoboinik, Lev; Balas, Shulamit; Amiel, Merav; Oz, Carla
Type: Technical Note
Published: 2022, Volume 72, Issue 2, Page 142
Abstract: The first steps in forensic DNA analysis are usually extraction and quantification of DNA. Quantification enables us to assess the efficiency of DNA extraction and the quality of extracted DNA. This technical note discusses the evaluation of two quantitative PCR (qPCR) protocols from QIAGEN: (1) Investigator Quantiplex Pro kit (Pro), which assesses the quantity and quality of DNA through amplification of long and short human autosomal targets and male DNA fragments using the TaqMan-based assay and (2) Investigator Quantiplex HYres kit (HYres), which detects and quantifies total human and male DNA using the Scorpion primer-based assay. In 69 male FTA reference samples, the male to human DNA concentration ratios were more closely correlated using the Pro kit than the HYres kit. Furthermore, reference samples (n = 80) amplified based on the quantification results showed a more uniform distribution of average peak height with the Pro kit than with the HYres kit. Of 318 casework samples, 23 samples indicated sub-threshold values when analyzed with the HYres kit, but quantification results of the same samples using the Pro kit indicated DNA amounts that crossed the threshold acceptable amplification. A complete STR DNA profile was obtained from only one of these samples. These results indicate that when choosing a quantification kit for a forensic case work laboratory, multiple considerations can contribute to successful workflow. Alongside the obvious advantages of a robust qPCR kit, the authors present additional considerations in choosing the right kit to obtain an optimal workflow in a casework forensic laboratory.

Visualization of Gunshot Residue on Dark Fabric: A Comparison of Infrared Photography and an Alternate Light Source

Author(s): Vecellio, Mark
Type: Technical Note
Published: 2022, Volume 72, Issue 2, Page 157
Abstract: Gunshot residue (GSR) is expelled from weapons upon discharge. GSR may be composed of smoke, metallic fragments, unburned and partially burned gunpowder particles, primer residues, and lubricants. Visible GSR deposition from gunpowder discharge may be useful to investigators in a variety of ways. GSR, however, may be obscured when it is deposited on dark surfaces. This study examines and compares two methods of visualizing obscured GSR deposited from two different firearms and types of ammunition from 3 inches, 9 inches, and 18 inches. Both infrared light and visible 450 nm blue light resulted in effective visualization of gunpowder discharge on black cotton fabric. Infrared, however, was more effective in revealing burning and scorching at the 3-inch range and bullet wipe at the 9- and 18-inch ranges. The 450 nm blue light was effective in revealing scattered particles, but ineffective in revealing burning, scorching, and bullet wipe. This research should be of value to investigators whose duties include searching for, collecting, and analyzing physical evidence.

WET UCIO -- New Powder Suspension Formula for Fingerprint Development on the Adhesive Side of Tape

Author(s): Claveria, Sergi; Clares, Néstor; Fernández, Patricia; Heredia, Roger; Godall, Anton
Type: Technical Note
Published: 2022, Volume 72, Issue 2, Page 174
Abstract: The development of fingerprints on the adhesive side of tape is a common task in forensic laboratories. Several methods are used for this purpose, although one of the most common is the application of powder suspension solutions. Our research has shown that it is possible to use a carbon-based powder suspension solution containing only two components (sodium lauryl sulphate and carbon black powder) in a simple, economical, and effective way. This solution was more effective than the commercial products WetWop and Adhesive-Side Powder Dark mixed with EZFLO Solution in the development of fingerprints on the adhesive side of eight different types of tapes.

Using Vacuum Metal Deposition to Detect Latent Fingermarks on Thermal Paper: A Pseudo-operational Trial

Author(s): Gabrielle Illston-Baggs, Gabrielle; Deacon, Paul; Nichols-Drew, Leisa; Farrugia, Kevin J.
Type: Article
Published: 2022, Volume 72, Issue 2, Page 185
Abstract: This work presents a pseudo-operational study on thermal paper for the detection of latent fingermarks with vacuum metal deposition (VMD) and amino acid sensitive reagents (1,2-indanedione and ninhydrin) on 120 thermal receipts. Each receipt was cut in half and separated into two processing groups. Each group was processed by one of two sequential treatment processes. Both sides of all receipts (thermal and nonthermal side) were treated. Fingermarks developed by each process were counted and imaged. Both sequences started with the use of UV imaging. Process A then continued with VMD using gold and zinc (VMDAu/Zn), then VMD using silver (VMDAg), followed by two amino acid sensitive reagents (1,2 indanedione and ninhydrin). Process B used only the amino acid sensitive reagents. Process A increased the detection rate on the thermal side by approximately a factor of two and a half when compared to the VMD techniques. The VMD processes did not visualize all of the marks detected by UV; however, this was the case for the amino acid sensitive reagents. The use of VMD can be suitable for the detection of fingermarks on thermal paper in cases where the text needs to be retained; however, the use of 1,2-indanedione and ninhydrin, in sequence with VMD, will detect the highest number of marks.

Using Silver Nitrate and Ultraviolet Light to Enhance Footwear Impressions Containing Salt Residue

Author(s): Elayas, Malak; Borsodi, Matthew; Nugent, Kimberly; Hamid, Desiree
Type: Article
Published: 2022, Volume 72, Issue 2, Page 200
Abstract: There is great interest in the identification of chemical reagents for the routine enhancement of footwear impressions. We present a silver nitrate recipe and application method, in combination with ultraviolet (UV) light, for the visual enhancement of footwear impressions containing salt residues on various substrates. A preliminary evaluation of various silver nitrate concentrations revealed that the best silver nitrate formulation, of the six that were tested, was 5% silver nitrate in 10% methanol. Three different salt brands were used to create residue impressions on six different substrates (ceramic tile, vinyl tile, Plexiglas, hardwood, nylon carpet, and concrete). Variables that were evaluated included the salt type and concentration, drying time, and substrate. An additional trial was conducted to expand on the previous results by enhancing impressions on ceramic tile, vinyl tile, untreated wood, polyester mat, and an olefin rug. The solution’s ability to enhance randomly acquired characteristics was also tested. Overall, a simple method of application of 5% silver nitrate in 10% methanol followed by exposure to a UV Polilight for 30 seconds enabled the visualization of the impression samples. This enabled increased visualization of class and randomly acquired characteristics that were present in the footwear impressions. We anticipate this formulation of silver nitrate becoming a useful, less toxic alternative for the visual enhancement of footwear impressions containing salt.

Modifying the Enhancement Capabilities of Luminol in Detecting Bloody Footwear Impressions using Highlighter Inks and Chemical Dyes

Author(s): Mahadeo, Natasha; Knaap, Wade; Gapinska-Serwin, Agata
Type: Article
Published: 2022, Volume 72, Issue 2, Page 225
Abstract: The blue luminescence of luminol is widely recognized, and luminol is known for its ability to detect the presence of blood by its peroxidase-like mechanism. This study was conducted to determine whether using highlighter ink or fluorescent dyes could improve certain aspects of luminol’s ability to detect and enhance bloody footwear impressions This study yielded positive results with fluorescein and the yellow and green Sharpie highlighter inks. The addition of the yellow and green inks allowed for the intensity of the reaction to be consistent over varying amounts of visible blood and under different lighting. We also found that the addition of fluorescein to the luminol formulation was most successful in not only enhancing the variables of intensity and duration of the luminescence, but also in visualizing the reaction without complete darkness. Additionally, fluorescein changed the blue color associated with luminol, allowing for better contrast with the substrate, thus providing useful applications to the field of forensic identification.

Back to Basics

Author(s):Siegel, Sandy, CLPE
Back to Basics
2022, Volume 72, Issue 2, Page 242
Funny finds from all over in their own words.

Issue 1: January - March 2022 

Letter to the Editor

Author(s): Kent, Terence
Type: Letter to the Editor
Published: 2022, Volume 72, Issue 1, Page 001
Abstract: Re: Evaluation of the Effectiveness of Lumicyano in the Recovery of Latent Prints When Compared to Rhodamine 6G Liquid Dye Stain Journal of Forensic Identification 2021, 71 (4), 2021

Probing the Formation and Characteristics of Downward Cast-off and Cessation Patterns

Author(s): Phua, Zai Rong; Hauw, Jie Boon
Type: Technical Note
Published: 2022, Volume 72, Issue 1, Page 004
Abstract: In bloodstain pattern analysis, the classification of bloodstains is key to shedding light on the mechanisms involved in bloodletting events. In this study, an experimental device that could swing forward and downward was constructed to produce the characteristics and quantitative information from the resultant patterns. The bloodstains were observed to be distributed in broadly three regions: The upper and central regions had spatter stains progressively changing in shape from circular to elliptical down the target, in a linear or curvilinear fashion, primarily as a result of cast-off. The lower region had spatter stains of less than 1 mm, usually deposited in a horizontal spread because of the cessation effect. We found that the height of the patterns increased with the swing speed and volume of blood that was used. In our experimentation, a high-speed camera proved to be useful in facilitating the visualization of the swing dynamics and bloodletting process. This provided a better understanding of the release of blood from an object’s end (formation of ligament and droplets) when surface tension effects were overcome.

Who Actually Discovered Fingerprint Powders?

Author(s): Claveria, Sergi
Type: Article
Published: 2022, Volume 72, Issue 1, Page 022
Abstract: Dr. René Forgeot is usually given credit for the discovery of using powder to develop fingerprints. However, this paper provides a review of documentation from the late 19th century and early 20th century and reveals that the first person who actually used powder for fingerprint development may have been Alphonse Bertillon.

Features of the Friction Ridge Skin: Attributes, Diagnosticity, and Limitations

Author(s): White, Alice V.
Type: Article
Published: 2022, Volume 72, Issue 1, Page 033
Abstract: The volar surface of hands and feet display an array of diagnostic features that exhibit both macroscopic and microscopic morphometric attributes. The macroscopic attributes are typically developmentally stable in the human population, which means these features can be exploited to determine which part of the hand or foot is represented in an impression and the distal orientation of an impression. The microscopic attributes are often subject to stochastic effects during embryological development or acquisition after birth. As a result, these smaller features can be combined with the macroscopic features to determine the ultimate utility of an impression and support source conclusions. Although the diagnosticity of each macroscopic and microscopic feature has theoretical constraints based on the influence of developmental stability and developmental noise, there are also biological constraints to the longevity of each feature and sources of distortion that affect the recording of the features in an impression. This article sheds light on the various features of the friction ridge skin, the attributes of each feature, the expected usefulness of these features for establishing search parameters, the expected usefulness in establishing identity, and common sources of variation in appearance of each feature. This article also illuminates areas where additional research would benefit the friction ridge community.

Back to Basics

Author(s):Siegel, Sandy, CLPE
Type: Back to Basics
Published: 2022, Volume 72, Issue 1, Page 130
Abstract: Funny finds from all over in their own words.